 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : PCR-based Restriction Fragment Length Polymorphism for Subtyping of Salmonella from Chicken Isolates) ผู้เขียน: Han Yu Jong, Pak Thae Su, พรรณนที แสนพงษ์, วรวิทย์ วัชชวัลคุ, Thavajchai Sukpuaram,  ดร.ปฐมาพร เอมะวิศิษฎ์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractGenotypic diversity in two flagellin genes, fliC and fljB, encoding phase-1 and phase-2 flagellin of Salmonella enterica, offers a potential biomarker for Salmonella subtyping. Forty-seven Salmonella isolates of 20 different serovars derived from chicken samples in Thailand were studied using the fliC/ fljB PCR-based RFLP assay. With two restriction endonucleases, MboI and HhaI, the fliC showed 11 and 9 patterns, while the fljB showed 6 and 7 patterns respectively. Though the PCR-based RFLP test cannot replace serotyping, the assay is based on the flagellin genes encoding proteins on the bacterial surface that are related to serotyping scheme. Overall, the assay was reproducible and successfully applied to simply screen Salmonella serovars as an alternative subtyping test for rapid traceability of Salmonella contamination in chicken production. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Antimicrobial Resistance of Campylobacter jejuni Isolated from Chicken in Nakhon Pathom Province, Thailand) ผู้เขียน:จณัญญา สุขเพสน์, ดร.ปฐมาพร เอมะวิศิษฎ์, รองศาสตราจารย์, วรวิทย์ วัชชวัลคุ, ดร.อรินทิพย์ ธรรมชัยพิเนต, ศาสตราจารย์ , Thavajchai Sukpuaram สื่อสิ่งพิมพ์:pdf AbstractCampylobacter jejuni isolated from retail market-chicken in Nakhon Pathom province were determined for resistance to quinolone and other antimicrobial agents by broth microdilution method. Sixty-eight C. jejuni strains were resistant to quinolone drugs including nalidixic acid (69.12%), norfloxacin (69.12%), ciprofloxacin (58.82%) and marbofloxacin (25.00%). High proportions of the isolates were resistant to tetracycline (77.94%), sulfamethoxazole (72.06%), kanamycin (51.47%), ampicillin 47.06% and streptomycin (42.65%). Low proportions of the isolates were found resistance to gentamycin (16.18%) and erythromycin (13.23%). Nearly 97% of the isolates were multiple resistances to more than 4 antimicrobial agents tested. |